Most available expression vectors are capable of introducing one copy of a cDNA, shRNA or other oligonucleotide fragments into bacterial or animal cells. Most multiple copy expression vectors inexistence utilize strategies of disconnected cDNAs under the control of separate promoters. Problems with other multiple copy vector systems include non-directional ligation, cDNA and vector self-ligation, inefficient cloning leading to cumbersome bacterial colony screening. 

To make the multi-copies, each cloning cycle includes many protocols as follows:  digest sample with restriction enzyme > electrophoresis to separate DNA > reclaim DNA from agarose > ligation > transformation of bacteria > select clone and expand > DNA extraction >digestion > identify with electrophoresis > expand the positive clone > purification of plasmid > digestion > identify with electrophoresis.  The total processing time takes 6-7 days.

The novel Viagene Ultra Ex vectors have a structure which can fix it on the wall of a tube. It causes the recombination to be extremely easy.  The new vector can make multiple copies and cause recombination only between vector and DNA fragments and avoid self-ligation.  The antibiotic selection is suitable for all prokaryote and eukaryote cell systems.  In addition, one vector can carry more than one copy of the same or different cDNAs combinations. This unique vector system can enhance the output of DNA, promote gene expression or raise the gene suppression level.

The new method is simple, saves on time and labor, and can increase substantially the success rate of cloning of multiple nucleotide fragments.

 Because Ultra-ex vector kits permit only one kind of correct ligation, the self-ligation of vectors or DNA fragments is unable to occur, and thus the probability of a positive clone is virtually 100%.

1.   Compared with other multi-copy vectors (7.7kbp ) on the market, the novel Viagene Biotech Ultra-EX vectors are small (molecular weight which is only 1.9kbp). They can bring more and the larger DNA fragments into the bacterium or cell.

2.   The single vector can carry more than one nucleic acid piece and thus can express multiple copies of the same DNA or multiple different cDNAs or shRNAs.

3.   The new vector is fixed on the wall of tube. By this method, making the multi-copies is extremely simple and rapid, saving time and expense.

4.   It can obviously enhance the output of DNA, promote gene expression or raise the gene suppression level.

5.   The recombination is directional and self-ligation is unable to occur. The probability of obtaining positive clones is nearly 100%.

UltraEx-B1 kit  [Cat#：VCTR001]

Because of the unique design, one vector can make and carry 10 gene expression recombinants. It can obviously enhance the output of DNA, promote gene expression or raise the level of gene suppression. It is suitable for all prokaryotic and eukaryotic cell system by Zeocin selection.

UltraEx-B2 kit [Cat#：VCTR002] 

Because of the unique design, one vector can make and carry 10 gene expression recombinants. It can obviously enhance the output of DNA, promote gene expression or raise the level of gene suppression. It is suitable for all prokaryotic and eukaryotic cell system by blasticidin selection.